Across the street from me

asparagus!

― Aaron Grossman (aajjgg), Sunday, 8 December 2002 04:49 (twenty-two years ago)

Analysis of dCLOCK protein sequence and mRNA expression. (A) Alignment of Drosophila and mouse CLOCK proteins, showing percent identity (similarity) between bHLH, PAS A, and PAS B regions. The extent of the PAS A and PAS B domains shown are as defined by the PAS-PYP module (36). The glutamine-rich (Q-rich) region contains polyglutamine (poly Q) repeats indicated in black. (B) Northern blot analysis of polyadenylated (poly A) mRNA from whole flies (lane 1), total RNA extracted from S2 cells (lane 2), and total head RNA (lane 3), hybridized with a riboprobe covering nucleotides 530 to 761 (37). Ten micrograms of RNA was loaded in each well. Because of the small difference in size, the known splice variants could not be distinguished in this assay. (C) Time course of dclock mRNA levels. RNase protection analysis was done on head RNA from flies harvested throughout a light-dark cycle (38). The white and black bars beneath the figure represent the periods of light and dark, respectively, with lights on at ZT0 and lights off at ZT12. Lane A is a positive control for splice variant A, which gives rise to the upper doublet encoding the full-length protein. Lane B is a control for variant B. The probe hybridizing to this variant is clipped at a two-base mismatch and gives rise to the lower doublet encoding truncated proteins. The relevant bands are marked with an arrowhead. The band in the lower panel is a protection of RP49 RNA for reference. The zeitgeber time is indicated above the lanes. RNA from appendages (App.), bodies (Body), and S2 cells is also shown. (D) Variant A (squares) and B (triangles) dclock RNA levels from Fig. 1C were normalized to RP49 values and plotted with zeitgeber time on the x axis. Light and dark periods are reversed relative to Fig. 1C to highlight the bimodality. The assays were performed three times with indistinguishable results on independently obtained samples.

― Dan I., Sunday, 8 December 2002 04:50 (twenty-two years ago)

Damn! I my non sequitur got beaten to the punch.

― Dan I., Sunday, 8 December 2002 04:51 (twenty-two years ago)

yes but yours was better!

― Aaron Grossman (aajjgg), Sunday, 8 December 2002 04:53 (twenty-two years ago)

Aaron you lovely zeitgeber.

― Dan I., Sunday, 8 December 2002 04:57 (twenty-two years ago)

what is zeitgeber?

― Aaron Grossman (aajjgg), Sunday, 8 December 2002 04:58 (twenty-two years ago)

Giver of time..but does that make sense?

― B, Sunday, 8 December 2002 05:32 (twenty-two years ago)

And in the building, is the woman with the golden face! Sh e screams like Jesus and the armies of red bastards fly from hell into my soul!

― Mike Hanle y (mike), Sunday, 8 December 2002 05:54 (twenty-two years ago)

pah Hanle y! That happens every flipping day to me!

― jel -- (jel), Sunday, 8 December 2002 10:15 (twenty-two years ago)

I love flipping day! What are you doing for this year's ?

― Mike Hanle y (mike), Monday, 9 December 2002 02:35 (twenty-two years ago)

http://bitchcakes.topcities.com/images/humpty.jpg

― Ally (mlescaut), Monday, 9 December 2002 02:41 (twenty-two years ago)

Analysis of dCLOCK protein sequence and mRNA expression. (A) Alignment of Drosophila and mouse CLOCK proteins, showing percent identity (similarity) between bHLH, PAS A, and PAS B regions. The extent of the PAS A and PAS B domains shown are as defined by the PAS-PYP module (36). The glutamine-rich (Q-rich) region contains polyglutamine (poly Q) repeats indicated in black. (B) Northern blot analysis of polyadenylated (poly A) mRNA from whole flies (lane 1), total RNA extracted from S2 cells (lane 2), and total head RNA (lane 3), hybridized with a riboprobe covering nucleotides 530 to 761 (37). Ten micrograms of RNA was loaded in each well. Because of the small difference in size, the known splice variants could not be distinguished in this assay. (C) Time course of dclock mRNA levels. RNase protection analysis was done on head RNA from flies harvested throughout a light-dark cycle (38). The white and black bars beneath the figure represent the periods of light and dark, respectively, with lights on at ZT0 and lights off at ZT12. Lane A is a positive control for splice variant A, which gives rise to the upper doublet encoding the full-length protein. Lane B is a control for variant B. The probe hybridizing to this variant is clipped at a two-base mismatch and gives rise to the lower doublet encoding truncated proteins. The relevant bands are marked with an arrowhead. The band in the lower panel is a protection of RP49 RNA for reference. The zeitgeber time is indicated above the lanes. RNA from appendages (App.), bodies (Body), and S2 cells is also shown. (D) Variant A (squares) and B (triangles) dclock RNA levels from Fig. 1C were normalized to RP49 values and plotted with zeitgeber time on the x axis. Light and dark periods are reversed relative to Fig. 1C to highlight the bimodality. The assays were performed three times with indistinguishable results on independently obtained samples.

Okay, but what does this have to do with the reactions between asparagine (cf. Aaron) and simple sugars in temperatures of 120C and higher?

― j.lu (j.lu), Monday, 9 December 2002 03:26 (twenty-two years ago)

Well it clearly explains the funny smell when one pees.

― Dan I., Sunday, 13 April 2003 23:37 (twenty-two years ago)